Establishment of ex vivo calibration curve for X-ray induced “dicentric + ring” and micronuclei in human peripheral lymphocytes for biodosimetry during radiological emergencies, and validation with dose blinded samples

In the modern developing society, application of radiation has increased extensively. With significant improvement in protection practices, exposure to human could be minimized substantially, but cannot avoided completely. Assessment is essential for regulatory decision and medical management as applicable. Until now, cytogenetic changes have served surrogate marker been extensively employed biological dose estimation various planned unplanned exposures. Dicentric Chromosomal Aberration (DCA) specific considered gold standard, micronucleus not very an alternative method biodosimetry. this study response curves were generated X-ray induced “dicentric + ring” micronuclei, lymphocytes three healthy volunteers [2 females (age 22, 23 years) 1 male (24 year)]. The blood samples irradiated with using LINAC (energy 6 MV, rate Gy/min), range 0–5Gy. Irradiated cultured processed harvest metaphases, per standard procedures recommended by International Atomic Energy Agency. Pooled data obtained from all volunteers, agreement Poisson distribution ring”, however over dispersion was observed micronuclei. Data (“dicentric micronuclei) fitted linear quadratic model expression YC αD βD2 Dose Estimate software, version 5.2. fit resulted coefficient α = 0.0006 (±0.0068) cell−1 Gy−1 β 0.0619 (±0.0043) Gy−2 0.0459 ± (0.0038) micronuclei 0.0185 (0.0010) respectively. Background frequencies 0.0004 0.0077 0.0012 cell−1, Established validated, reconstructing doses 8 blinded (4 DCA 4 CBMN) coefficients here. Estimated within variation 0.9–16% 21.7–31.2% These established potential biodosimetry occupational, clinical accidental exposures, initial triage management.